Quantitative optical measurement of mitochondrial superoxide dynamics in pulmona

Reactive oxygen species (ROS) play a vital role in cell signaling and redox regulation, but when present in excess, lead to numerous pathologies. Detailed quantitative characterization of mitochondrial superoxide anion (O;-2 production in fetal pulmonary artery endothelia cells (PAECs) has never been reported. The aim of this study is to assess mitochondrial O;-2 production in cultured PAECs over time using a novel quantitative optical approach. The rate, the sources, and the dynamics of O;-2 pr