Fluorescence Quenching of Pheophytin a by Copper(II) Ions

A method was developed for determination of Cu(II) ions quantitatively by measuring fluorescent intensity of pheophytin-a (Pheoa) solution. The Pheoa was obtained by de-intercalation of magnesium from the porphyrin ring of chlorophyll-a (Chla) extracted from fresh spinach leaves. Its two UV-Vis abso